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Apr . 12, 2024 16:26 Back to list

Cpv Ccv Ag Test Kit Instructions

    Canine coronavirus antigen detection kit is a rapid immunochromatographic detection technology product for detecting the presence of canine coronavirus antigen in dog feces and rectal samples. Canine coronavirus (CCV) is a single-stranded RNA virus, which contains 6~7 kinds of polypeptides, 4 of which are glycopeptides, and does not contain RNA polymerase and neuraminidase. The virus infection can cause gastroenteritis symptoms in dogs with different degrees, mainly manifested as frequent vomiting, diarrhea, depression, anorexia, and other symptoms. Canine coronavirus infection can occur all year round, but it is more common in winter. Sick dogs are the main source of infection, which can be transmitted through the respiratory tract, digestive tract, feces, and pollutants.

    In order to detect and control the spread of the virus in time, this kit was developed to detect whether canine coronavirus antigen is contained in dog feces and rectal samples simply and quickly. The reaction principle of the kit is based on the anti-CCV monoclonal antibody labeled with colloidal gold. When CCV antigen exists in the sample, they will combine with the antibody on the detection line, thus presenting a burgundy band in the detection window. However, if there is no CCV antigen in the sample, there will be no color reaction.

    The reagent box contains a test card, diluent, and disposable cotton swabs. When in use, just take a sample of dog feces or rectum, add it to the diluent, stir, and wait for a certain time, then suck the diluted sample with a straw and drop it into the sample adding a hole in the detection card. After that, wait for 10-15 minutes at room temperature to interpret the results. Negative results will only show a purple-red control line (C), while positive results will show purple-red bands of both the detection line (T) and the control line (C). The test results after more than 20 minutes will be considered invalid.

    In order to ensure the accuracy and reliability of the test results, there are certain requirements for sample collection and processing. The test samples should be fresh and put into the diluent for processing as soon as possible. If it cannot be detected in time, the diluted sample can be stored for a period of time under appropriate conditions (2-8℃ or -20℃). At the same time, when testing at different temperatures, it is necessary to adjust the interpretation time according to the environmental temperature.

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