Over the past decades, paper-based lateral flow immunoassays (LFIAs) have been extensively developed for rapid, facile, and low-cost detection of a wide array of target analytes in a point-of-care manner. Conventional home pregnancy tests are the most significant example of LFAs, which detect elevated concentrations of human chorionic gonadotrophin (hCG) in body fluids to identify early pregnancy.

A pregnancy test is used to determine whether a female is pregnant or not. The two primary methods are testing for the female pregnancy hormone (human chorionic gonadotropin (hCG)) in blood or urine using a pregnancy test kit, and scanning with ultrasonography.[1] Testing blood for hCG results in the earliest detection of pregnancy.[2] Almost all pregnant women will have a positive urine pregnancy test one week after the first day of a missed menstrual period.[3]

Dilute urine samples (i.e. with a low specific gravity) may not contain a representative levels of hCG, potentially leading to a false negative result. If pregnancy is suspected, collect an early morning (first) urine sample and retest.

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